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MassChrom® 25-OH-Vitamin D3/D2 in serum/plasma (LC-MS/MS)



Kit MassChrom® 25-OH-Vitamin D3/D2 in serum/plasma (LC-MS/MS)

• Suitable for every tandem-MS with sufficient sensitivity
• Isotopically labelled internal standard
• 3PLUS1® multilevel calibrator set
• Online sample preparation
• Minimal ion suppression effects

Vitamin D has been widely recognised as a biomarker in relation to osteoporosis and osteopenia. It has also been shown to play a prominent role in immune response, cardiovascular disease and certain types of cancer. Vitamin D analysis, therefore, carries significance in several respects. 

25-OH-vitamin D3 constitutes the principal diagnostic target and is the main metabolite of vitamin D in humans; it is the precursor of the physiologically most active compound 1,25-(OH)2-vitamin D3. Vitamin D deficiency is treated with vitamin D3 or vitamin D2 supplementation. Vitamin D2 is easier to produce synthetically and, where prescribed, its monitoring is also necessary.

This reagent kit allows for the specific analysis of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum and plasma by tandem mass spectrometry. Manual sample preparation is minimised and limited to simple and effective protein precipitation. A subsequent online trap column concentrates the analytes and separates interfering substances. A six-port-valve connects the trap column to an HPLC column which provides further purification. The use of atmospheric pressure chemical ionisation (APCI) and an isotopically labelled internal standard assures precision and robustness and minimises ion suppression effects. The 3PLUS1® multilevel calibrators offer additional precision.


MassChrom® 25-OH-Vitamin D3/D2 in serum/plasma (LC-MS/MS)

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TipoDescripciónReferencia
Kit
Kit de análisis de la 25-OH-vitamina D3 / D2 en suero/plasma MassChrom®. Para 200 análisis.
CH-62000
Componente
Fase A móvil, 1000 ml
CH-62001
Componente
Fase B móvil, 1000 ml
CH-62002
Componente
Solución de lavado, 1000 ml.
CH-62009
Componente
Reactivo de precipitación
CH-62003
Componente
Estándar interno, 40 ml.
CH-62004
Componente
Viales de reacción, 100 unidades.
CH-3006
Accesorio
Kit Calibrador de Plasma multinivel 3PLUS1 ® 25-OH-vitamina D3/D2, 4 x 1 ml.
CH-62028
Accesorio
Columna Trap, 1 unidad.
CH-62110
Accesorio
Columna Analitica, 1 unidad.
CH-62100
Accesorio
Tuning Mix 25-OH-Vitamin D3 / D2, 2 ml
CH-62015
Control
Control de Plasma, niveles I y II, de 25-OH-Vitamina D2/D3, 2 x 5 x 1 ml.
CH-0221
Control
Control de Plasma, nivel I, de 25-OH-Vitamina D2/D3, 5 x 1 ml.
CH-0222
Control
Control de Plasma, nivel Ii, de 25-OH-Vitamina D2/D3, 5 x 1 ml.
CH-0223