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Gel Y PCR



ATPTM Gel/PCR Fragment DNA Extraction Kit is designed to recover or concentrate DNA fragments (50 bp-10 kb) from agarose gel, PCR or other enzymatic reaction. The method uses a chaotropic salt, guanidine thiocyanante, to dissolve the agarose gel and denature enzymes. The DNA fragments in the chaotropic salt are then bound to the uniquely designed matrix of glass-fiber in the spin column (1) in the optimized salt concentration and pH provided by our buffer. Whereas unwanted impurities, such as salts, enzymes, primers unincorporated nucleotides, dyes, and ethidium bromide flow through the column and are easily and efficiently removed from reaction mixture. After washing step, the purified DNA fragments are eluted by low-salt Elution Buffer or water. The entire procedure does not require DNA phenol extraction and alcohol precipitation, and could be completed in 20 minutes.

Format: Spin column
Sample: Up to 300 mg agarose gel slice; Up to 100 µl PCR product or other enzymatic reaction
Operation: Centrifuge / vacuum manifold
DNA size: 50 bp ~ 10 kb
Operation time: 20 minutes for gel extraction; 15 minutes for PCR clean up
Expectant recovery: 75-85% for gel extraction; 85-95% for PCR clean up
Application: DNA Sequencing; Ligation; PCR;Restriction Digestion; DNA labeling

ATP™ Gel/PCR DNA Fragments Extraction Kit is designed to recover or concentrate DNA fragments ( 50 bp-10 kb ) from agarose gel, PCR or other enzymatic reaction.
 
1.  The method uses a chaotropic salt,guanidine thiocyanante , to dissolve the agarose gel and denature enzymes. 
2.  The DNA fragments in the chaotropic salt are then bound to the uniquely designed glass fiber matrix in the spin column in the optimized salt concentration and pH provided by our buffer.
3.  Whereas unwanted impurities, such as salts, enzymes, primers unincorporated nucleotides, dyes, and ethidium bromide flow through the column and are easily and efficiently removed from reaction mixture.
4.  After washing off contaminants, the purified DNA fragments are eluted by a low salt elution buffer or water. 

The entire procedure does not require DNA phenol extraction and alcohol precipitation , and could be completed in 20 minutes .

The quality of ATP™ Gel/PCR fragments Extraction Kit is tested on a lot-to-lot basis. The efficiency of DNA recovery is tested by isolation of DNA fragment of various sizes from either aqueous solution or agarose. The purified DNA is checked by agarose gel analysis.

Gel / PCR DNA Extraction Kit
96-Well Gel/PCR DNA Extraction Kit
Fragment DNA Binding Column


Gel Y PCR

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DescripciónReferencia
Gel/PCR DNA Kit extracción de fragmentos 100 prep
ATP-ADF100
Gel/PCR DNA Kit extracción de fragmentos 300 prep
ATP-ADF300
Gel/PCR 96-Well DNA Extraccion Kits 4 placas
ATP-ADF9604
Gel/PCR 96-Well DNA Extraction Kits 10 placas
ATP-ADF9610
SEQ Dye 96-Well Clean Up Kit 4 placas
ATP-ADC9604
SEQ Dye 96-Well Clean Up Kit 10 placas
ATP-ADC9610
Fragment DNA Binding Columna 50 pcs/bolsa
ATP-DFC50