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Tosoh TSK Fase Reversa



This mode of HPLC, often referred to as RPLC or RPC, has been largely responsible for the widespread popularity of HPLC as a chromatographic technique. The opposite of normal phase chromatography, reversed phase liquid chromatography requires a non-polar stationary phase and a polar mobile phase. Typically the mobile phase consists of a mixture of water (buffer) and acetonitrile, methanol, THF, or 2-propanol.
Silica particles are most commonly used as the support, which then is derivatized with octadecylsilane (ODS). Polymer-based supports have been introduced as an alternative to silica-based reversed phase columns, particularly for analyzing basic compounds in their neutral state at high pH.
RPC columns can be applied to the analysis of a wide variety of compounds, ranging from neutral polar and non-polar solutes to acidic, basic, and amphoteric compounds. RPC is also an efficient technique for the analysis of derivatized amino acids, peptides and proteins, although protein structure is not always maintained due to the high concentration of organic solvent required for their elution.

80 Å Monomeric RP Silica
100 Å Monomeric RP Silica
2 µm Super Series RP Silica
Specialty RP Silica
High Throughput RP Silica

Tosoh TSK Fase Reversa