|
|
|
|
|
|
|
|
|
|
MassChrom® 25-OH-Vitamin D3/D2 in serum/plasma (LC-MS/MS)
Kit MassChrom® 25-OH-Vitamin D3/D2 in serum/plasma (LC-MS/MS)
• Suitable for every tandem-MS with sufficient sensitivity
• Isotopically labelled internal standard
• 3PLUS1® multilevel calibrator set
• Online sample preparation
• Minimal ion suppression effects
Vitamin D has been widely recognised as a biomarker in relation to osteoporosis and osteopenia. It has also been shown to play a prominent role in immune response, cardiovascular disease and certain types of cancer. Vitamin D analysis, therefore, carries significance in several respects.
25-OH-vitamin D3 constitutes the principal diagnostic target and is the main metabolite of vitamin D in humans; it is the precursor of the physiologically most active compound 1,25-(OH)2-vitamin D3. Vitamin D deficiency is treated with vitamin D3 or vitamin D2 supplementation. Vitamin D2 is easier to produce synthetically and, where prescribed, its monitoring is also necessary.
This reagent kit allows for the specific analysis of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum and plasma by tandem mass spectrometry. Manual sample preparation is minimised and limited to simple and effective protein precipitation. A subsequent online trap column concentrates the analytes and separates interfering substances. A six-port-valve connects the trap column to an HPLC column which provides further purification. The use of atmospheric pressure chemical ionisation (APCI) and an isotopically labelled internal standard assures precision and robustness and minimises ion suppression effects. The 3PLUS1® multilevel calibrators offer additional precision.
|
|
|
|
MassChrom® 25-OH-Vitamin D3/D2 in serum/plasma (LC-MS/MS)
|
|
|
|
|
|
|
|
|