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TOYOPEARL Reactive Affinity Resins

TOYOPEARL Reactive Affinity Resins require activation prior to ligand attachment.

 

The following TOYOPEARL Reactive Affinity Resins are currently available from Tosoh Bioscience:

 

TOYOPEARL AF-Amino-650M

TOYOPEARL AF-Carboxy-650M

TOYOPEARL AF-Formyl-650M

TOYOPEARL AF-Amino-650M
TOYOPEARL AF-Carboxy-650M
TOYOPEARL AF-Formyl-650M

TOYOPEARL AF-Amino-650M is a reactive resin that requires activation for ligand attachment. The resin may be used to couple ligands using their carboxyl groups through peptide bond formation or aldehyde groups by reductive amination. Aldehyde groups may be present in a carbohydrate or glycoprotein ligand or may be introduced into the ligand by mild, periodate oxidation.

 

AFC_Amino_structure.jpg

TOYOPEARL AF-Carboxy-650M is a reactive resin that requires activation for ligand attachment. It provides mild coupling to amino groups of proteins or low molecular weight ligands. The carbodiimide mediated coupling reaction produces an amide bond between ligand and support.

 

AFC_Carboxy_structure.jpg

TOYOPEARL AF-Formyl-650 is a reactive resin that requires activation for ligand attachment. Ligands may be coupled to the resin under mild conditions exclusively using primary amines. The ligand is bound to the resin by a stable secondary amine linkage. A wide variety of industrial enzymes have been immobilized on aldehyde-bearing supports. Consistent aldehyde content and physical properties are ensured from batch to batch.

 

AFC_Formyl_structure.jpg

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