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Halo

High Speed Separations

  • Smaller reduced plate heights lead to high efficiencies; narrower and taller peaks, for improved resolution and lower detection limits (LODs and LOQs)
  • Flat van Deemter plot and higher linear velocity optimum allow higher flow rates with minimal column efficiency loss

High Resolution Separations

  • High efficiency with longer geometries (100, 150, 250 mm) provides greater resolving power for challenging applications
  • Lower back pressure permits columns to be used in series for the most demanding UHPLC and HPLC separations

Excellent Ruggedness and Reproducibility

  • Less plugging, longer usable column lifetime and greater uptime due to larger porosity frits (vs. sub-2 µm totally porous (non-core) columns)
    • 2 µm frits for HALO 2.7 and HALO 5
    • 1 µm frits for HALO 2 vs. 0.2–0.5 µm frits for sub-2 µm non-core columns
  • Excellent column-to-column and lot-to-lot reproducibility
HALO Fused-Core Performance
Fused-Core silica particles
Performance vs. non-core Columns
Applications
Downloads

HALO Fused-Core Performance

High Speed Separations

  • Smaller reduced plate heights lead to high efficiencies; narrower and taller peaks, for improved resolution and lower detection limits (LODs and LOQs)
  • Flat van Deemter plot and higher linear velocity optimum allow higher flow rates with minimal column efficiency loss

High Resolution Separations

  • High efficiency with longer geometries (100, 150, 250 mm)
    provides greater resolving power for challenging applications
  • Lower back pressure permits columns to be used in series for the most demanding UHPLC and HPLC separations

Excellent Ruggedness and Reproducibility

  • Less plugging, longer usable column lifetime and greater uptime due to larger porosity frits (vs. sub-2 µm totally porous (non-core) columns)
    • 2 µm frits for HALO 2.7 and HALO 5
    • 1 µm frits for HALO 2 vs. 0.2–0.5 µm frits for sub-2 µm non-core columns
  • Excellent column-to-column and lot-to-lot reproducibility
Tecnología Fused Core
de sílice de HALO Protein 1000Å 2,7 µm
Scanning Electron Microscope (SEM) image of HALO 2 particles. The particle size distribution is very narrow due to the separate sizing steps for solid cores and finished Fused-Core silica particles.
SEM image of a focused-ion-beam-cleaved HALO Protein 1000Å 2.7 µm silica particle. This “cut-away” view shows the solid core with its outer porous layer.

Van Deemter Plot of Plate Height vs. Linear Velocity (flow rate)

Van Deemter Plot of Plate Height vs. Linear Velocity (flow rate)

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Advanced Materials Technology

HALO Guard columns