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YMC

RP Bioseparation Columns

Chromatographers know the problems during method development: “Which phase is suitable and provides a simple and robust separation?” In the field of biochromatography, phase selection is a key to success!.

Ion Exchange (IEX) Columns

Ion exchange chromatography (IEX) is widely used for analysis and purification of biomolecules. YMC-BioPro ion exchange columns are specifically designed for separation of proteins, peptides and nucleic acids.

Size Exclusion (SEC) Columns

YMC-Pack Diol is available in four tightly controlled porosities and is, therefore, suitable for separation and molecular weight determination of a wide range of peptides, proteins, oligonucleotides, carbohydrates and other biopolymers with molecular weights of approx 200 to several hundred thousand.

Hydrophobic Interaction (HIC) Columns

BioPro HIC BF is designed and optimised for analysis and possibly lab-scale purification of antibodies, antibody-drug conjugates and other proteins. Optimised surface modification and column packing method realize high separation performance.

YMC offers columns packed with non-bonded silica or with silica gel modified with polar groups.

 

Application Database

 

YMC Calculators

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RP Bioseparation Columns
Ion Exchange (IEX) Columns
C4 and C8 Selectivities for Proteins and Peptides
C18 Selectivities for Proteins and Peptides
C18 and C8 Selectivities for Oligonucleotides
Specifications
RP columns for bioseparations
Ion Exchange (IEX) Columns
YMC SEC Columns
BioPro HIC

YMC Biochromatography columns   YMC Oligonucleotide Columns  YMC Triart u-HPLC   YMC Accura Triart   YMC-U-HPLC Troubleshooting  Selector de Columnas Bio YMC

C4 and C8 Selectivities for Proteins and Peptides
YMC-Triart Bio C4*
YMCbasic
Modification
C4 (USP L26)
equivalent to C8 (USP L7)
Particle Size [µm]
1, 9, 3, 5 µm
3, 5 µm
Pore Size [nm / Å]
30 nm / 300 Å
20 nm / 200 Å
Surface Area [m²/g]
-
175 m²/g
pH Range
1.0 - 10.0
2.0 - 7.5
C18 Selectivities for Proteins and Peptides
YMC-Triart C18*
YMC-Triart Bio C18*
Meteoric Core C18 BIO
Modificación
C18 (USP L1)
C18 (USP L1)
C18 (USP L1)
Tamaño Partícula [µm]
1, 9, 3, 5 µm
1, 9, 3, 5 µm
2.7 µm
Tamaño Poro [nm / Å]
12 nm / 120 Å
30 nm / 300 Å
16 nm / 160 Å
Área Superficie [m²/g]
360 m²/g
-
90 m²/g
Rango pH
1.0 - 12.0
1.0 - 12.0
1.5 - 10.0
C18 and C8 Selectivities for Oligonucleotides
YMC-Triart C18*
YMC-Triart Bio C18*
YMC-Triart C8*
Hydrosphere C18
Modification
C18 (USP L1)
C18 (USP L1)
C8 (USP L7)
C18 (USP L1)
Particle Size [µm]
1, 9, 3, 5 µm
1, 9, 3, 5 µm
1, 9, 3, 5 µm
2, 3, 5 µm
Pore Size [nm / Å]
12 nm / 120 Å
30 nm / 300 Å
12 nm / 120 Å
12 nm / 120 Å
Surface area [m²/g]
360 m²/g
-
360 m²/g
330 m²/g
pH Range
1-12
1-12
1-12
2-8
BioPro IEX
BioPro IEX QA
BioPro IEX SP
BioPro IEX QF
BioPro IEX SF
Matrix
Hydrophilic polymer (polymethacrylate)
Hydrophilic polymer (polymethacrylate)
Hydrophilic polymer (polymethacrylate)
Hydrophilic polymer (polymethacrylate)
Particle Size [µm]
5 µm
5 µm
3, 5 µm
3, 5 µm
Pore Size [nm / Å]
100 nm / 1000 Å
100 nm / 1000 Å
Non-porous
Non-porous
Charged Group
-CH2N+(CH3)3
-(CH2)3SO3-
-CH2N+(CH3)3
-(CH2)3SO3-
Counter Ion
-Cl-
-Na+
Cl-
Na+
Ion-exchange Capacity
0.075-0.100 meq/ml-resin
0.070-0.095 meq/ml-resin
0.075-0.110 meq/ml-resin
0.230-0.290 meq/ml-resin
Dynamic binding capacity
110-150 mg BSA/ml-resin
70-100 mg human IgG/ml-resin
12-20 mg BSA/ml-resin
10-18 mg human IgG/ml-resin
pH Range
2.0 - 12.0
2.0 - 12.0
2.0 - 12.0
2.0 - 12.0
YMC-Triart Bio C4*
YMCbasic
Modification
C4 (USP L26)
equivalent to C8 (USP L7)
Particle Size [µm]
1, 9, 3, 5 µm
3, 5 µm
Pore Size [nm / Å]
30 nm / 300 Å
20 nm / 200 Å
Surface Area [m²/g]
-
175 m²/g
pH Range
1.0 - 10.0
2.0 - 7.5
YMC-Triart C18*
YMC-Triart Bio C18*
Meteoric Core C18 BIO
Modification
C18 (USP L1)
C18 (USP L1)
C18 (USP L1)
Particle Size [µm]
1, 9, 3, 5 µm
1, 9, 3, 5 µm
2.7 µm
Pore Size [nm / Å]
1.2, 12 nm / 12, 120 Å
3, 30 nm / 30, 300 Å
1.6, 16 nm / 16, 160 Å
Surface Area [m²/g]
360 m²/g
-
90 m²/g
pH Range
1.0 - 12.0
1.0 - 12.0
1.5 - 10.0
C18 and C8 Selectivities for Oligonucleotides
YMC-Triart C18*
YMC-Triart Bio C18*
YMC-Triart C8*
Hydrosphere C18
Modification
C18 (USP L1)
C18 (USP L1)
C8 (USP L7)
C18 (USP L1)
Particle Size [µm]
1, 9, 3, 5 µm
1, 9, 3, 5 µm
1, 9, 3, 5 µm
2, 3, 5 µm
Pore Size [nm / Å]
12 nm / 120 Å
30 nm / 300 Å
12 nm / 120 Å
12 nm / 120 Å
Surface area [m²/g]
360 m²/g
-
360 m²/g
330 m²/g
pH Range
1-12
1-12
1-12
2-8
YMC-Pack SIL (Silica)
YMC-Pack PVA-Sil
YMC-Pack CN (Cyano)
YMC-Pack Diol-NP
YMC-Pack Polyamine II
YMC-Pack NH2 (Amino)
YMC-Pack TMS (C1)
Modification
Silica (USP L3)
Polyvinyl alcohol (USP L24)
Cyanopropyl (USP L10)
1,2-Dihydroxypropyl (USP L20)
Sec./ tert. amines (USP L111)
Aminopropylsilane (USP L8)
Trimethylsilane (USP L13)
Particle Size [µm]
3, 5 µm
5 µm
3, 5 µm
5 µm
5 µm
3, 5 µm
3, 5 µm
Pore Size [nm / Å]
6, 12, 20, 30 nm / 60, 120, 200, 300 Å
12 nm / 120 Å
12, 30 nm / 120, 300 Å
6, 12 nm / 60, 120 Å
12 nm / 120 Å
12 nm / 120 Å
12, 30 nm / 120, 300 Å
Surface area [m²/g]
450, 330, 175, 100 m²/g
330 m²/g
330, 175 m²/g
450, 330 m²/g
330 m²/g
330 m²/g
330, 175 m²/g
pH Range
2-7.5
2-9.5
2-7.5
2-7.5
2-7.5
2-7.5
2-7.5

YMC-RP columns

The C18 column with 12 nm pore size is suitable for small peptides. The highest efficiency for large peptides or small proteins can be obtained by employing a C8 phase characterised by a 20 nm pore size. Also, most proteins or even antibodies are eluted efficiently with a C4 column with 30 nm porosity.

However, the separation may also be influenced by the hydrophobicity of the peptide/protein and the nature of the column’s bonded phase. 

Metal-free columns are available for improved sensitivity and peak shape of coordinating compounds such as nucleotides or oligonucleotides.

Ion exchange chromatography (IEX) is widely used for analysis and purification of biomolecules. BioPro ion exchange columns are specifically designed for separation of proteins, peptides and nucleic acids.
BioPro IEX columns are based on porous and non-porous hydrophilic polymer beads with low nonspecific adsorption. They also show higher binding capacity and higher recovery of biomolecules compared to conventional IEX columns. The completely spherical, monodisperse beads, together with optimal packing technology, provide high theoretical plate numbers and symmetrical peak shapes!

YMC-Pack Diol is available in four tightly controlled porosities and is, therefore, suitable for separation and molecular weight determination of a wide range of peptides, proteins, oligonucleotides, carbohydrates and other biopolymers with molecular weights of approx 200 to several hundred thousand.

YMC-SEC MAB is especially designed for the analysis of antibodies, their fragments and aggregates ro provide high resolution for the high and low molecular weight range.

Furthermore, YMC offers a wide range of column dimensions suitable for analytical to preparative scale separations.

The silica is derivatised with 1,2-dihydroxypropane and, with the variety of pore size available, allows analyses over a broad range of peptide to large proteins or antibodies.

BioPro HIC HT as well as BioPro HIC BF are based on a non-porous hydrophobic polymer. They are designed and optimised for analysis and lab-scale purification of antibodies, antibody-drug conjugates and other proteins.

Optimised surface modification and column packing method realise high separation performance. High-throughput analysis with a faster flow rate can be achieved together with a high resolution using BioPro HIC HT. The rigid particle provides higher pressure tolerance compared to other HIC columns, which allows higher flow rates and therefore shorter analysis times.

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Length
10 mm (10)
100 mm (190)
125 mm (13)
15 mm (1)
150 mm (186)
20 mm (9)
250 mm (58)
30 mm (32)
300 mm (1)
33 mm (17)
5 mm (42)
50 mm (178)
75 mm (101)
Column ID
0,50 mm (1)
1,0 mm (20)
10 mm (15)
100 µm (32)
2,0 mm (53)
2,1 mm (142)
20 mm (24)
3,0 mm (78)
30 mm (27)
300 µm (100)
4,0 mm (40)
4,6 mm (170)
500 µm (100)
6,0 mm (3)
75 µm (32)
8,0 mm (3)
Technology
HIC (4)
Hilic (15)
IEX (31)
Normal Phase/Hilic (2)
Reverse Phase (773)
Reverse Phase/Hilic (15)
SFC (3)
Support
Fused Core Silica (15)
Hybrid (531)
Polymeric (12)
Silica (285)
Brand
BioPro HIC (4)
BioPro IEX (31)
YMC Hydrosphere (149)
YMCbasic (106)
YMC-Meteoric Core (15)
YMC-Triart (535)
Function
C18 (263)
C18 AQ (149)
C18 ExRS (15)
C4 (22)
C8 (315)
Diol-HILIC (15)
Pentafluorophenyl propyl (15)
Phenyl butyl (15)
Polyamine (2)
Quaternary Amine (10)
Quaternary Amine / Sulfopropyl (1)
Sulfopropyl (20)
Type
Analytical (172)
Capillary (291)
Microbore (176)
Nanobore (63)
Narrowbore (75)
Preparative (55)
Semipreparative (10)
Particle Size
1,9 µm (106)
10 µm (8)
2,3 µm (2)
2,7 µm (15)
3 µm (314)
4 µm (2)
5 µm (376)
6 µm (5)
Proprietary (1)
Pore Size
120 Å (636)
160 Å (15)
200 Å (6)
300 Å (36)
80 Å (15)
NON-POROUS (1)
Proprietary (1)
Reference YMC-BA99S03-0346WT
Description YMCbasic, Classical Analytical HPLC Column (4.6 mm i.d.), S-3 µm, 30 x 4.6 mm
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Reference YMC-BA99S03-0504QC
Description YMCbasic, Classical Analytical HPLC Cartridge (4.0 mm i.d.), S-3 µm, 50 x 4.0 mm
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Reference YMC-BA99S03-0504WT
Description YMCbasic, Classical Analytical HPLC Column (4.0 mm i.d.), S-3µm, 50 x 4.0 mm
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Reference YMC-BA99S03-0546WT
Description YMCbasic, Classical Analytical HPLC Column (4.6 mm i.d.), S-3 µm, 50 x 4.6 mm
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Reference YMC-BA99S03-05E8AU
Description YMCbasic, Capillary column 1/1 n 1/16", S-3 µm, 50 x 0.075 mm
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Reference YMC-BA99S03-05F0AU
Description YMCbasic, Capillary column 1/1 umn 1/16", S-3 µm, 50 x 0.1 mm
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Reference YMC-BA99S03-05H0AU
Description YMCbasic, Capillary column 1/1 umn 1/16", S-3 µm, 50 x 0.3 mm
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Reference YMC-BA99S03-05H0RU
Description YMCbasic, Capillary column 1/3 umn 1/32", S-3 µm, 50 x 0.3 mm
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Reference YMC-BA99S03-05J0AU
Description YMCbasic, Capillary column 1/1 umn 1/16", S-3 µm, 50 x 0.5 mm
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Reference YMC-BA99S03-05J0RU
Description YMCbasic, Capillary column 1/3 umn 1/32", S-3 µm, 50 x 0.5 mm
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Reference YMC-BA99S03-1001WT
Description YMCbasic, Narrowbore HPLC colu mn (1.0 mm i.d.), S-3 µm, 100 x 1.0 mm
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Reference YMC-BA99S03-1003WT
Description YMCbasic, Microbore HPLC colum n (3.0 mm i.d.), S-3 µm, 100 x 3.0 mm
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Reference YMC-BA99S03-1004QC
Description YMCbasic, Classical Analytical HPLC Cartridge (4.0 mm i.d.), S-3 µm, 100 x 4.0 mm
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Reference YMC-BA99S03-1046WT
Description YMCbasic, Classical Analytical HPLC Column (4.6 mm i.d.), S- 3 µm, 100 x 4.6 mm
To use the Favorites List you must be registered Sign up
Reference YMC-BA99S03-10E8AU
Description YMCbasic, Capillary column 1/1 1/16", S-3 µm, 100 x 0.075 mm
To use the Favorites List you must be registered Sign up
Reference YMC-BA99S03-10F0AU
Description YMCbasic, Capillary column 1/1 mn 1/16", S-3 µm, 100 x 0.1 mm
To use the Favorites List you must be registered Sign up
Reference YMC-BA99S03-10H0AU
Description YMCbasic, Capillary column 1/1 mn 1/16", S-3 µm, 100 x 0.3 mm
To use the Favorites List you must be registered Sign up
Reference YMC-BA99S03-10H0RU
Description YMCbasic, Capillary column 1/3 mn 1/32", S-3 µm, 100 x 0.3 mm
To use the Favorites List you must be registered Sign up
Reference YMC-BA99S03-10J0AU
Description YMCbasic, Capillary column 1/1 mn 1/16", S-3 µm, 100 x 0.5 mm
To use the Favorites List you must be registered Sign up
Reference YMC-BA99S03-10J0RU
Description YMCbasic, Capillary column 1/3 mn 1/32", S-3 µm, 100 x 0.5 mm
To use the Favorites List you must be registered Sign up
showing 20 of 844 products