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Columnas Tosoh

TSKgel Chromatography Columns
Tosoh Bioscience offers a complete line of TSKgel (U)HPLC columns for use with conventional and UHPLC systems to meet your purification needs. The TSKgel product line includes columns in nearly all HPLC modes, including Size Exclusion, Ion Exchange, Reversed Phase, Hydrophobic Interaction, Affinity and Normal Phase/HILIC.

 

Chromatography mode Types of compounds analysed
Size Exclusion Chromatography (SEC) " Natural (biopolymers), synthetic polymers
Ion Exchange Chromatography (IEC) "
Charged compounds
Reversed Phase Chromatography (RPC) " Relatively hydrophobic compounds,
neutrals, weak acids, weak bases
Hydrophobic Interaction Chromatography (HIC) "
Hydrophobic biomolecules
Hydrophilic Interaction Chromatography (HILIC) "
Highly polar compounds
Affinity Chromatography (AFC) " Carbohydrates, proteins, specific antigens

 



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Reversed Phase Liquid Chromatography (RPC)
Hydrophilic Interaction Chromatography (HILIC)
Ion Exchange Chromatography (IEX)
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Reversed Phase Liquid Chromatography (RPC) has become an accepted tool for the separation of peptides, proteins and other biopolymers in the pharmaceutical, chemical and biochemical industries.
Reversed Phase Liquid Chromatography (RPC) separates molecules based on nonpolar interactions and requires a nonpolar stationary phase and a polar mobile phase. Typically the mobile phase consists of a mixture of water (buffer) and acetonitrile, methanol, THF, or 2-propanol.

 

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Normal phase and hydrophilic interaction liquid chromatography (HILIC) are primarily used to separate polar and hydrophilic compounds. In reversed phase mode very polar compounds are often not sufficiently retained in low percent organic, or even in 100% aqueous mobile phase. The order of elution in normal phase is opposite that found in reversed phase for the same mixture of compounds. Although non-polar organic mobile phases and a silica stationary phase were used traditionally in normal phase LC, today most separations are performed with aqueous-organic mobile phases and a more polar-bonded stationary phase. This mode of HPLC is now commonly referred to as HILIC, hydrophilic interaction liquid chromatography.

By using an amide or amino bonded phase column, polar compounds can be retained by a normal phase or hydrophilic interaction chromatography retention mechanism. Typical mobile phases in HILIC are aqueous buffers with organic modifiers – primarily acetonitrile. In contrast to the retention behavior in reversed phase, in HILIC, solutes will be retained longer when increasing the percent acetonitrile.

 

 

 

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Separation in Ion Exchange chromatography (IEC or IEX) is based on reversible adsorption of charged solute molecules to immobilized functional groups of opposite charge. Biomolecules generally have charged groups on their surfaces, which change with the buffer pH. Elution can be accomplished by changing the ionic strength or the pH, of which changing the ionic strength by increasing the salt concentration is most common.
IEX is further subdivided into cation exchange and anion exchange chromatography. Anion and cation exchange phases are classified as strong or weak, depending on how much the ionization state of the functional groups vary with pH. A strong ion exchange phase has the same charge density on its surface over a broad pH range, whereas the charge density of a weak ion exchange phase changes with pH, affecting its selectivity, which differs at different pH values.

 

 

 

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Anion Exchange (3)
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Amide (18)
CN (2)
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SS (65)
Reference TH-21867
Description Amide-80 3 µm silica 4,6mm ID  15cm L
Price 1.062,31€
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Reference TH-21938
Description TSKgel ODS-100V,ODS-100V, 3 um , 100 Å silica 2,0 mm ID 10 cm L
Price 640,00€
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Reference TH-21939
Description TSKgel ODS-100V,ODS-100V, 3 um , 100 Å silica 3,0 mm ID 10 cm L
Price 640,00€
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Reference TH-21940
Description TSKgel ODS-100V,ODS-100V, 3 um , 100 Å silica 4,6 mm ID 10 cm L
Price 640,00€
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Reference TH-21967
Description TSKgel NH2-100, 3 ?m, 100 Å NE W! silica 2,0 mm ID 5 cm L
Price 758,85€
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Reference TH-21968
Description TSKgel HILIC COLUMNS , NH2-100 , 3 µm, 100 Å        , silica, 2 mm ID, 15 cm L
Price 841,92€
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Reference TH-21969
Description TSKGEL NH2-100 3um 4.6mm ID x 5cm
Price 758,85€
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Reference TH-21970
Description TSKgel NH2-100 3um 4,6mm ID x 15,0cm L
Price 841,92€
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Reference TH-21982
Description Amide-80 HR, 5um, 80A silica, 4.6mm ID x 25cm
Price 1.132,69€
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Reference TH-21999
Description TSKgel NH2-100 DC,3um,,100Å dir ect coupling 2D LC to other HPLC column silica 4.6mmID 5cm L
Price 813,08€
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Reference TH-22700
Description TSKgel ODS-100V, 3 um,, 100 Å s ilica 2,0 mm ID 2 cm L
Price 389,62€
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Reference TH-22701
Description TSKgel ODS-100V, 3 um,, 100 Å s ilica 2,0 mm ID 25 cm L
Price 732,31€
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Reference TH-22702
Description TSKgel ODS-100V, 3 um,, 100 Å s ilica 3,0 mm ID 2 cm L
Price 389,62€
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Reference TH-22703
Description TSKgel ODS-100V, 3 um,, 100 Å s ilica 3,0 mm ID 3,5 cm L
Price 507,31€
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Reference TH-22704
Description TSKgel ODS-100V, 3 um,, 100 Å s ilica 3,0 mm ID 25 cm L
Price 732,31€
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Reference TH-22705
Description TSKgel ODS-100V, 3 um,, 100 Å s ilica 4,6 mm ID 2 cm L
Price 389,62€
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Reference TH-22706
Description TSKgel ODS-100V, 3 um,, 100 Å s ilica 4,6 mm ID 3,5 cm L
Price 507,31€
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Reference TH-22707
Description TSKgel ODS-100V, 3 um,, 100 Å s ilica 4,6 mm ID 25 cm L
Price 732,31€
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Reference TH-22708
Description TSKgel ODS-100V, 5 um,, 100 Å,  3 pieces/pkg needs cartridge holder silica 2,0 mm ID 1 cm L
Price 656,15€
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Reference TH-22709
Description TSKgel ODS-100V, 5 um,, 100 Å s ilica 2,0 mm ID 2 cm L
Price 333,08€
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showing 100 of 160 products