The Purest Reagents for Separation Optimisation in HPLC
Although reversed phase HPLC is one of the most important techniques in chromatography, by itself it is limited to separations of non-ionic (neutral) and non-polar compounds.
Polar or ionic substances tend to separate badly on reversed phase systems. So, bearing in mind that many compounds of biological interest are either ionic or highly polar, what can the HPLC analyst do to improve such separations? Either give up or reach for ROMIL-SpR™ Super Purity Reagents. The addition to the mobile phase of one of these high purity substances, as a counter-ion of opposite charge, enables the sample species to form a neutral 'ion pair' that will now readily interact with the reversed phase packing and thereby permit a clean separation.
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