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Tosoh

Tosoh Bioscience TSKgel columns for UHPLC are used for the analysis and purification of proteins, peptides, biopolymers and low molecular weight compounds. We provide UHPLC columns for many chromatographic modes such as hydrophobic/hydrophilic interaction, ion exchange, reversed phase, and affinity chromatography

For over 30 years TSKgel SW-type silica-based columns have been the biopharmaceutical industry’s standard in gel filtration chromatography of biomolecules.

TSKgel columns are known for their reliability and suitability for a variety of chromatographic applications. The packings in the columns are either silica-based or polymeric-based material, in particle sizes ranging from 2 µm to 20 µm. Columns are available in analytical to preparative sizes, in stainless steel, PEEK®, or glass.

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Size Exclusion Chromatography (SEC)
Ion Exchange Chromatography (IEX)
Reversed Phase Liquid Chromatography (RPC)
Hydrophobic Interaction Chromatography (HIC)
FcR Affinity

Size Exclusion Chromatography (SEC) separates molecules based on their size, or more precisely, their hydrodynamic volume. It is based on the discrimination of individual sample components by the pores of the packing material. Large sample molecules cannot or can only partially penetrate the pores and elute from the column first, whereas smaller molecules can access all or a larger number of pores and elute later. SEC is the only mode of chromatography that does not involve interaction with a stationary phase by means of adsorption or partitioning of the solutes.
The terms SEC, GFC (gel filtration chromatography) and GPC (gel permeation chromatography) all refer to the same chromatographic technique. In GFC an aqueous mobile phase is used, while an organic mobile phase is employed in GPC. The general term SEC covers both uses.

 

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Separation in Ion Exchange chromatography (IEC or IEX) is based on reversible adsorption of charged solute molecules to immobilized functional groups of opposite charge. Biomolecules generally have charged groups on their surfaces, which change with the buffer pH. Elution can be accomplished by changing the ionic strength or the pH, of which changing the ionic strength by increasing the salt concentration is most common.
IEX is further subdivided into cation exchange and anion exchange chromatography. Anion and cation exchange phases are classified as strong or weak, depending on how much the ionization state of the functional groups vary with pH. A strong ion exchange phase has the same charge density on its surface over a broad pH range, whereas the charge density of a weak ion exchange phase changes with pH, affecting its selectivity, which differs at different pH values.

 

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Reversed Phase Liquid Chromatography (RPC) has become an accepted tool for the separation of peptides, proteins and other biopolymers in the pharmaceutical, chemical and biochemical industries.
Reversed Phase Liquid Chromatography (RPC) separates molecules based on nonpolar interactions and requires a nonpolar stationary phase and a polar mobile phase. Typically the mobile phase consists of a mixture of water (buffer) and acetonitrile, methanol, THF, or 2-propanol.

 

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Hydrophobic interaction chromatography (HIC) is based on non-polar interactions that are induced by high salt mobile phases. Stationary phases are similar to reversed phase chromatography (RPC) but the density of functional groups is lower. A weakly non-polar stationary phase is used with an aqueous mobile phase containing a high concentration of a chaotropic salt.
The technique is mainly applied to the separation of proteins, which are eluted by decreasing the salt concentration or by adding a low percentage of organic solvent. Although also based on hydrophobic interactions, selectivity in HIC separations is distinctly different from that in reversed phase chromatography. Despite the lower peak capacity in HIC compared to RPC, HIC has the advantage that the mobile phase conditions (primarily aqueous) do not usually disrupt higher-order protein structures.

 

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TSKgel FcR-IIIA columns are based on a recombinant Fc gamma IIIa receptor which is immobilized as a ligand on polymer particles. They separate monoclonal antibodies according their affinity to the receptor and enable a fast analysis of biological activity as well as fractionation along with biological activity.

 

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Choose your product
showing 17 of 17 products
Technology
Anion Exchange (2)
Cation Exchange (1)
HIC (2)
Reverse Phase (1)
SEC (11)
Support
PW (7)
Silica (4)
SW (6)
Function
Butyl (2)
DEAE (1)
DIOL (4)
DNA-NPR (1)
Octadecyl (1)
SP (1)
UP-SW (4)
Pore Size
125 Å (2)
300 Å (2)
NON-POROUS (6)
Type
Column (13)
Guard Column (4)
Particle Size
2 µm (4)
2,5 µm (6)
3 µm (3)
Length
10 cm (1)
15 cm (2)
150 mm (2)
20 mm (4)
3,5 cm (4)
30 cm (1)
300 mm (2)
7,5 cm (1)
Column ID
4,6 mm (16)
6,0 mm (1)
Material
SS (13)
Reference TH-13075
Description DEAE-NPR, 2.5 µm, non-porous polymer 4,6 mm 3,5 cm
Price 1.113,00€
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Reference TH-13076
Description SP-NPR, SS, 2.5 µm, non-porous polymer 4,6 mm 3,5 cm
Price 1.113,00€
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Reference TH-14005
Description Octadecyl-NPR, SS, 2.5 µm, non -porous 4,6 mm x 3,5 cm th-15022
Price 1.015,00€
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Reference TH-14947
Description Butyl-NPR, SS, 2.5 µm, non-por ous polymer 4,6 mm 3,5 cm
Price 1.113,00€
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Reference TH-18249
Description DNA-NPR, 2.5 µm, non-porous polymer 4,6 mm 7,5 cm
Price 1.689,00€
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Reference TH-22792
Description TSKgel PW Column SuperOligo PW , 3 µm, polymer, 6 mm ID X 15  cm L
Price 1.890,00€
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Reference TH-23448
Description UP-SW3000 2 µm, 4.6 x 300 mm ,  silica, 4,6 mm ID, 30 cm L
Price 2.453,00€
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Reference TH-23449
Description UP-SW3000 2 µm, 4.6x150 mm, si lica, 4,6 mm ID, 15 cm L
Price 1.822,00€
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Reference TH-23514
Description UP-SW2000 2 um, 4.6 x 300 mm
Price 2.463,00€
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Reference TH-23515
Description UP-SW2000 2 µm, 4.6 x 150 mm
Price 1.832,00€
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Reference TH-23516
Description UP-SW2000 Guardcolumn 4.6 x 20  mm
Price 597,00€
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Reference TH-23517
Description UP-SW2000 Guardcolumn DC 4.6 x  20 mm
Price 597,00€
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Reference TH-23524
Description UP-SW Aggregate 3 um, 4.6 x 300 mm
Price 2.463,00€
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Reference TH-23525
Description UP-SW Aggregate 3 um,  4.6 x 150 mm
Price 1.832,00€
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Reference TH-23526
Description UP-SW Aggregate Guardcolumn  4.6x20 mm
Price 597,00€
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Reference TH-23527
Description UP-SW Aggregate DC Direct Conn ect Guardcol. 4.6x20 mm
Price 597,00€
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Reference TH-42168
Description TSKgelPW-HICCOLUMN Butyl-NPR,  2.5 µm, non-porous, polymer, 4 ,6 mm ID X 10 cm L
Price 1.392,00€
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showing 17 of 17 products