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Columnas Tosoh

TSKgel Chromatography Columns
Tosoh Bioscience offers a complete line of TSKgel (U)HPLC columns for use with conventional and UHPLC systems to meet your purification needs. The TSKgel product line includes columns in nearly all HPLC modes, including Size Exclusion, Ion Exchange, Reversed Phase, Hydrophobic Interaction, Affinity and Normal Phase/HILIC.

 

Chromatography mode Types of compounds analysed
Size Exclusion Chromatography (SEC) " Natural (biopolymers), synthetic polymers
Ion Exchange Chromatography (IEC) "
Charged compounds
Reversed Phase Chromatography (RPC) " Relatively hydrophobic compounds,
neutrals, weak acids, weak bases
Hydrophobic Interaction Chromatography (HIC) "
Hydrophobic biomolecules
Hydrophilic Interaction Chromatography (HILIC) "
Highly polar compounds
Affinity Chromatography (AFC) " Carbohydrates, proteins, specific antigens

 



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Reversed Phase Liquid Chromatography (RPC)
Hydrophilic Interaction Chromatography (HILIC)
Ion Exchange Chromatography (IEX)

Reversed Phase Liquid Chromatography (RPC) has become an accepted tool for the separation of peptides, proteins and other biopolymers in the pharmaceutical, chemical and biochemical industries.
Reversed Phase Liquid Chromatography (RPC) separates molecules based on nonpolar interactions and requires a nonpolar stationary phase and a polar mobile phase. Typically the mobile phase consists of a mixture of water (buffer) and acetonitrile, methanol, THF, or 2-propanol.

 

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Normal phase and hydrophilic interaction liquid chromatography (HILIC) are primarily used to separate polar and hydrophilic compounds. In reversed phase mode very polar compounds are often not sufficiently retained in low percent organic, or even in 100% aqueous mobile phase. The order of elution in normal phase is opposite that found in reversed phase for the same mixture of compounds. Although non-polar organic mobile phases and a silica stationary phase were used traditionally in normal phase LC, today most separations are performed with aqueous-organic mobile phases and a more polar-bonded stationary phase. This mode of HPLC is now commonly referred to as HILIC, hydrophilic interaction liquid chromatography.

By using an amide or amino bonded phase column, polar compounds can be retained by a normal phase or hydrophilic interaction chromatography retention mechanism. Typical mobile phases in HILIC are aqueous buffers with organic modifiers – primarily acetonitrile. In contrast to the retention behavior in reversed phase, in HILIC, solutes will be retained longer when increasing the percent acetonitrile.

 

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Separation in Ion Exchange chromatography (IEC or IEX) is based on reversible adsorption of charged solute molecules to immobilized functional groups of opposite charge. Biomolecules generally have charged groups on their surfaces, which change with the buffer pH. Elution can be accomplished by changing the ionic strength or the pH, of which changing the ionic strength by increasing the salt concentration is most common.
IEX is further subdivided into cation exchange and anion exchange chromatography. Anion and cation exchange phases are classified as strong or weak, depending on how much the ionization state of the functional groups vary with pH. A strong ion exchange phase has the same charge density on its surface over a broad pH range, whereas the charge density of a weak ion exchange phase changes with pH, affecting its selectivity, which differs at different pH values.

 

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showing 20 of 187 products
Technology
Anion Exchange (3)
Cation Exchange (3)
Hilic (21)
Reverse Phase (99)
Support
PW (6)
Silica (132)
Function
Amide (18)
Amide-80 (4)
CN (2)
NH2 (3)
Octadecyl (96)
Octyl (4)
Phenyl (2)
QC (1)
SAX (1)
SCX (2)
Sugar AXG (1)
Sugar AXI (1)
TMS (1)
Pore Size
100 Å (72)
110 Å (4)
120 Å (10)
125 Å (1)
250 Å (1)
60 Å (5)
80 Å (24)
Type
Column (150)
Guard Column (20)
Guardgel (14)
Particle Size
10 µm (10)
2,3 µm (4)
3 µm (45)
5 µm (71)
8 µm (1)
Length
0,4 cm (2)
1 cm (7)
1,5 cm (6)
10 cm (15)
15 cm (26)
2 cm (11)
25 cm (24)
3,5 cm (11)
30 cm (9)
5 cm (14)
7,5 cm (14)
Column ID
1,0 mm (1)
2,0 mm (36)
21,5 mm (7)
3,0 mm (27)
3,2 mm (6)
4,0 mm (2)
4,6 mm (53)
6,0 mm (2)
7,8 mm (5)
Material
SS (78)
Reference TH-03410
Description Pre-Column Filter with 0.5µm S S Frit
Price 214,00€
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Reference TH-03411
Description Replacement 0.5µm SS Frits, fo r TH-03410
Price 127,00€
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Reference TH-06172
Description ODS-120A, SS, 10 µm, 120 Å silica 21,5 mm 30 cm
Price 3.519,00€
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Reference TH-06191
Description Endfitting w/ Fixed 10µm Frit,  for all 21.5mm ID S
Price 407,00€
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Reference TH-07124
Description ODS-120A, SS, 5 µm, 120 Å silica 4,6 mm 25 cm
Price 547,00€
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Reference TH-07125
Description ODS-120T, SS, 5 µm, 120 Å silica 4,6 mm 25 cm
Price 547,00€
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Reference TH-07129
Description ODS-120A, SS, 10 µm, 120 Å silica 7,8 mm 30 cm
Price 1.491,00€
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Reference TH-07130
Description ODS-120T,  SS, 10 µm, 120 Å silica 7,8 mm 30 cm
Price 1.491,00€
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Reference TH-07134
Description ODS-120T,  SS, 10 µm, 120 Å silica 21,5 mm 30 cm
Price 3.519,00€
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Reference TH-07156
Description SCX, SS, 5 µm polymer 6 mm 15 cm
Price 1.418,00€
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Reference TH-07157
Description SAX, SS, 5 µm polymer 6 mm 15 cm
Price 1.428,00€
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Reference TH-07158
Description SCX, SS, 5 µm polymer 7,8 mm 30 cm
Price 1.832,00€
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Reference TH-07190
Description TMS-250, 10 µm, 250Å silica 4,6 mm 7,5 cm
Price 695,00€
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Reference TH-07619
Description Endfitting w/ Fixed 2µm Frit,  for all 4.6mm ID SS 
Price 127,00€
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Reference TH-07636
Description ODS-120A, SS, 5 µm, 120 Å silica 4,6 mm 15 cm
Price 523,00€
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Reference TH-07637
Description ODS-120T,  SS, 5 µm, 120 Å silica 4,6 mm 15 cm
Price 523,00€
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Reference TH-08092
Description FITTING + FRITS FOR 08033 COLU MN
Price 127,00€
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Reference TH-08095
Description Endfitting w/ Fixed 2µm Frit,  for all 7.8mm ID SS 
Price 127,00€
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Reference TH-08148
Description ODS-80TM, SS, 5 µm, 80Å  silica 4,6 mm 15 cm
Price 452,00€
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Reference TH-08149
Description ODS-80TM, SS, 5 µm, 80Å  silica 4,6 mm 25 cm
Price 529,00€
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showing 20 of 187 products