GL Sciences’ MonoCap series, created synthetically via sol-gel method, and an octadecyl silane chemically bonded, has a very uniform three dimensional structure that shows excellent reproducibility from batch-to-batch. The solid structure of GL Sciences’ monolithic silica eliminates the need for frits or filters at the ends of the column, thereby reducing dead volume that might otherwise lead to band broadening or sample recovery. The high porosity of our monolithic silica allows high flow rates to be used without loss of resolution or creation of high operating pressure. An optimized balance of throughpores and mesopores provides the critically important combination of efficiency, separation speed, large volume sample-loading, and small volume sample-recovery.
MonoCap High Resolution provide extremely high efficiency, delivering over 200,000 plates for a 2,000 mm length column. The High Resolution Ultra type deliver over 300,000 plates. The Fast-flow type is compatible with high flow rate analysis due to its’ low flow resistance. In addition, the equilibration time can be minimized further by setting the flow rate high. MonoCap Nano-flow is a high-density monolithic capillary column offering extremely high sensitivity in LC/MS due to the optimization of mesopore and throughpore sizes. MonoCap Trap columns have a relatively big throughpore, which are available for on-line pre-concentration or desalting of protein and peptide samples prior to HPLC separation with mass spectrometry detection.