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Columnas Tosoh

TSKgel Chromatography Columns
Tosoh Bioscience offers a complete line of TSKgel (U)HPLC columns for use with conventional and UHPLC systems to meet your purification needs. The TSKgel product line includes columns in nearly all HPLC modes, including Size Exclusion, Ion Exchange, Reversed Phase, Hydrophobic Interaction, Affinity and Normal Phase/HILIC.

 

Chromatography mode Types of compounds analysed
Size Exclusion Chromatography (SEC) " Natural (biopolymers), synthetic polymers
Ion Exchange Chromatography (IEC) "
Charged compounds
Reversed Phase Chromatography (RPC) " Relatively hydrophobic compounds,
neutrals, weak acids, weak bases
Hydrophobic Interaction Chromatography (HIC) "
Hydrophobic biomolecules
Hydrophilic Interaction Chromatography (HILIC) "
Highly polar compounds
Affinity Chromatography (AFC) " Carbohydrates, proteins, specific antigens



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Reversed Phase Liquid Chromatography (RPC)
Hydrophilic Interaction Chromatography (HILIC)

Reversed Phase Liquid Chromatography (RPC) has become an accepted tool for the separation of peptides, proteins and other biopolymers in the pharmaceutical, chemical and biochemical industries.
Reversed Phase Liquid Chromatography (RPC) separates molecules based on nonpolar interactions and requires a nonpolar stationary phase and a polar mobile phase. Typically the mobile phase consists of a mixture of water (buffer) and acetonitrile, methanol, THF, or 2-propanol.

 

More Information

Normal phase and hydrophilic interaction liquid chromatography (HILIC) are primarily used to separate polar and hydrophilic compounds. In reversed phase mode very polar compounds are often not sufficiently retained in low percent organic, or even in 100% aqueous mobile phase. The order of elution in normal phase is opposite that found in reversed phase for the same mixture of compounds. Although non-polar organic mobile phases and a silica stationary phase were used traditionally in normal phase LC, today most separations are performed with aqueous-organic mobile phases and a more polar-bonded stationary phase. This mode of HPLC is now commonly referred to as HILIC, hydrophilic interaction liquid chromatography.

By using an amide or amino bonded phase column, polar compounds can be retained by a normal phase or hydrophilic interaction chromatography retention mechanism. Typical mobile phases in HILIC are aqueous buffers with organic modifiers – primarily acetonitrile. In contrast to the retention behavior in reversed phase, in HILIC, solutes will be retained longer when increasing the percent acetonitrile.

 

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Choose your product
showing 19 of 19 products
Technology
Hilic (7)
Reverse Phase (11)
Support
Silica (19)
Function
Amide (6)
Octadecyl (8)
Octyl (2)
Phenyl (2)
Pore Size
110 Å (10)
Type
Column (6)
Guardgel (1)
Particle Size
2 µm (7)
2,3 µm (7)
3 µm (2)
4 µm (3)
Length
1 cm (2)
10 cm (7)
15 cm (2)
5 cm (8)
Column ID
1,0 mm (1)
2,0 mm (11)
2,1 mm (2)
3,0 mm (3)
4,6 mm (2)
Material
SS (7)
Reference TH-18154
Description Super ODS, 2 µm silica 4,6 mm 5 cm
Price 634,00€
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Reference TH-18197
Description Super ODS, 2 µm silica 4,6 mm 10 cm
Price 679,00€
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Reference TH-19541
Description Super ODS, 2 µm 5cm,2mm
Price 606,00€
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Reference TH-19542
Description Super ODS, 2 µm 10cm,2mm
Price 622,00€
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Reference TH-19672
Description Super-ODS Guardcolumn for P/N  19541 and 19542    N 1cm,2mm
Price 289,00€
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Reference TH-20013
Description TSKgel Super-Octyl,Super-Octyl , 2 µm, 110 Å silica 2,0 mm ID 5 cm L
Price 593,00€
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Reference TH-20014
Description TSKgel Super-Octyl,Super-Octyl , 2 µm, 110 Å silica 2,0 mm ID 10 cm L
Price 609,00€
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Reference TH-20015
Description TSKgel Super-ODS,Super-ODS, 2  um, 110 Å silica 1,0 mm ID 5 cm L
Price 616,00€
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Reference TH-20017
Description TSKgel Super-Phenyl,Super-Phen yl, 2 µm, 110 Å silica 2,0 mm ID 5 cm L
Price 593,00€
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Reference TH-20018
Description TSKgel Super-Phenyl,Super-Phen yl, 2 µm, 110 Å silica 2,0 mm ID 10 cm L
Price 609,00€
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Reference TH-21927
Description ODS-140HTP, 2,3 µm silica 2,1m m ID 5cm L
Price 647,00€
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Reference TH-21928
Description ODS-140HTP, 2,3 µm silica 2,1m m ID 10cm L
Price 661,00€
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Reference TH-23454
Description Amide-80, 2 µm, silica, 2 mm I D, 5 cm L
Price 825,00€
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Reference TH-23455
Description Amide-80, 2 µm , silica, 2 mm  ID, 10 cm L
Price 863,00€
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Reference TH-23456
Description Amide-80, 2 µm , silica, 2 mm  ID, 15 cm L
Price 953,00€
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Reference TH-23457
Description Amide-80, 2 µm , silica, 3 mm  ID, 5 cm L
Price 835,00€
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Reference TH-23458
Description Amide-80, 2 µm, silica, 3 mm I D, 10 cm L
Price 873,00€
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Reference TH-23459
Description Amide-80, 2 µm, silica, 3 mm I D, 15 cm L
Price 963,00€
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Reference TH-23460
Description Amide-80 2 µm (DC) Direct Conn ect Guardcolumn, silica, 2 mm ID, 1 cm L
Price 508,00€
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showing 19 of 19 products